General description
Unlike most TGF-β family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the related hyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes.
Biochem/physiol Actions
GDF-3 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| potency | 100-150 ng/mL |
| mol wt | 26.0 kDa |
| packaging | pkg of 20 μg |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| UniProt accession no. | Q9NR23 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... GDF3(9573) |
