TFCH，25MG，SML3746-25MG，Sigma

Biochem/physiol Actions

Brain penetrant, photo-stable, hydrazinyl coumarin compound that acts as a two-photon highly selective fluorescent sensor for both malondialdehyde and formaldehyde
TFCH is a photo-stable, hydrazinyl coumarin compound that acts as a two-photon fluorescent sensor for biomolecule carbonyls with low biotoxicity and extraordinary biocompatibility. TFCH is blood-brain barrier permeant, highly selective for both malondialdehyde (MDA) and formaldehyde (FA) with low detection levels (0.13 & 0.11 uM), respectively. It is shown to simultaneously and separately detect and image MDA and FA (0.11 uM) levels in living brains (0.37 mg/kg, i.p.) and in PC12 cells by two-photon microscopy. TFCH efficiently detects modest forms of renal injury with much greater sensitivity than standard assays for nephrotoxins. For MDA: Ex 365 & 800 nm, Em 440 nm; For FA: Ex 365 & 800 nm, Em 510 nm.