产品介绍
Product Description
The QIAprep& CRISPR Kit is an integral part or our QIAprep& CRISPR solutions workflow, which provides rapid and sensitive characterization of CRISPR-based genome editing events in adherent or suspension-cultured human, mouse or rat cells. The innovative system combines liquid-based sample preparation with downstream PCR detection of your region of interest using CRISPR-Q Custom PCR Assays and optional sequencing using CRISPR-Q Sanger Primers. The fast and simple workflow allows all-in-one processing and analysis in only a few steps and is compatible with different plate formats and cell types. The QIAprep& CRISPR Kit allows cell lysis preparation starting from down to 10 cells/µl and direct PCR detection from down to 1 cell. The CRISPR-Q Sanger Sequencing Analysis tool, which is available in GeneGlobe, enables quick and convenient analysis to complete the entire CRISPR editing detection workflow.
产品特点
Features
- Fast and simple workflow allows rapid and sensitive characterization of CRISPR-based genome editing events
- Compatible with adherent or suspension-cultured human, mouse or rat cells
- Cell lysis preparation starting from down to 10 cells/µl
- Sensitive detection from a wide cell input range supports various DNA editing scenarios
- Unique internal control detects and distinguishes sample or assay quality issues
产品应用
Applications
The QIAprep& CRISPR Kit and corresponding CRISPR-Q Custom PCR Assays and CRISPR-Q Sanger Primers are well-suited for practically any researcher who needs to characterize CRISPR-based editing events:
- Characterization of gene editing events
- Functional studies including gene knock-out or insertion
- Base editing
- Genome screening (CRISPRi libraries, reversible knockdowns)
