产品介绍
Product Description
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规格 |
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| Accession Number: | BC074796.2 |
| Assay Entry: | Cell-based beta lactamase reporter gene |
| Cell Line: | CHO-K1 |
| Cell State: | Dividing Cells |
| Druggable Target: | GPCRs |
| Gene Symbol: | HCRTR1 |
| Population Status: | Pool of Stable Cells |
| Product Line: | GeneBLAzer® |
| Reporter Gene: | BLA (Beta-Lactamase) |
| System Type: | GeneBLAzer™ |
| Target Entry: | HCRTR1 |
| Detection Method: | Fluorescent |
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储存 |
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(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 cells o GeneBLAzer ® HCRTR1 -NFAT-bla CHO-K1 Assay Protocol o Certificate of Analysis The GeneBLAzer® HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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描述 |
The GeneBLAzer® HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession # NM_001525) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.
The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
