规格
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Assay:
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β-Gal (lacZ) Assay |
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Compatible Cells:
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Mammalian Cells |
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Form:
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Liquid |
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High-throughput Compatibility:
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High-throughput Compatible |
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Label Type:
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Unlabeled |
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Product Line:
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Galacto-Light Plus™, NovaBright™ |
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Readout:
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End Point |
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Sample Type:
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Cell Cultures |
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Substrate:
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Galacton-Plus® |
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Substrate Properties:
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Chemical Substrate |
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Substrate Type:
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Beta-Gal Substrate |
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Target Enzyme:
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Beta-Galactosidase |
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Technique:
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Enhanced Chemiluminescence |
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Validated Application:
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Enzyme Assay |
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Detection Method:
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Chemiluminescent |
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Format:
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96-well plate |
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For Use With (Equipment):
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Luminometer (Microplate) |
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Quantity:
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15,000 assays |
储存
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This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C. |
The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.
• The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.
Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Ready-to-Use
The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.
Products described herein are guaranteed for 12 months from the date of purchase.
For Research Use Only. Not for use in diagnostics procedures.
