产品介绍
Product Description
EpiTect Fast Bisulfite Conversion Kits are a coordinated system of kits that enable highly efficient conversion of unmethylated cytosines to uracils in as little as 30 minutes while offering outstanding flexibility in choice of starting material. Streamlined procedures for sample lysis and direct bisulfite conversion of DNA from whole blood, cultured cells, tissue samples, genomic DNA and formalin-fixed, paraffin-embedded (FFPE) slices make efficient bisulfite conversion fast and effortless. Innovative DNA Protect technology ensures effective DNA denaturation without degradation, resulting in high DNA yield and reproducible results. The spin-column procedures can be automated on the QIAcube Connect. The procedure in 96-well format delivers ultrafast, high-throughput bisulfite conversion of DNA and can be processed using a centrifuge or vacuum manifold.
产品特点
Features
- Optimized procedure enables high cytosine conversion rates of over 99%
- Efficient bisulfite conversion in as little as 30 minutes
- Dedicated solutions for gDNA, whole blood, cells, tissue and FFPE slices
- Fast, streamlined procedure for efficient conversion and purification of DNA, also in 96-well plates
- Unique DNA Protect technology prevents DNA degradation
产品应用
Applications
The EpiTect Fast Bisulfite Conversion Kit delivers fast and efficient bisulfite conversion of DNA from various starting material that is highly suited for all techniques currently used for the analysis of DNA methylation, including:
- PCR: end-point, real-time and methylation-specific
- Bisulfite sequencing (direct and cloning)
- COBRA (Combined Bisulfite Restriction Analysis)
- Pyrosequencing
