规格
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Column Type:
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Affinity Column |
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Product Line:
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Sepharose® |
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Shipping Condition:
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Wet Ice |
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Stationary Phase:
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Protein G |
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Quantity:
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5mL |
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Format:
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Suspension |
储存
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This product is supplied as a 50% suspension (5 ml Recombinant Protein G-Sepharose® 4B + 5 ml PBS) |
Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose® 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.19% Kathon CG/ICP).
Applications: Immunoglobulin affinity purification and Immunoprecipitation.
Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:
• all four subclasses of human IgG
• mouse IgG
• rabbit IgG
• guinea pig IgG
• rat IgG
• goat IgG
• sheep IgG
• cow IgG
• horse IgG
Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.
