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规格 |
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| Exonuclease Activity: | 3' - 5', 5' - 3' |
| Fidelity (vs. Taq): | 6 X |
| GC-Rich PCR Performance: | Low |
| Hot Start: | Built-In Hot Start |
| Overhang: | Mixed |
| Polymerase: | Platinum® Taq DNA Polymerase High Fidelity |
| Product Line: | Platinum® |
| Quantity: | 100 reactions |
| Reaction Format: | Separate Components |
| Reaction Speed: | Standard |
| Shipping Condition: | Dry Ice |
| Size (Final Product): | 20 kb or less |
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储存 |
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• 1 x 20 µL Platinum Taq DNA Polymerase High Fidelity (5 U/µL) • 1 x 1.25 mL 10X High Fidelity Buffer [600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4] • 1 x 1 mL 50 mM MgSO4 Store at -10°C to -30°C. |
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描述 |
Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic 'hot-start' technology. Features of Platinum® Taq DNA Polymerase, High Fidelity:
• Fidelity—greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size—amplification of fragments up to 15 kb (see figure)
• Convenience—room temperature reaction assembly
• Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit definition
One unit of Platinum® Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
