产品介绍
Product Description
The EZ1&2 ccfDNA Kit provides easy and fully automated extraction of high-quality cfDNA from up to 8 ml of serum or plasma samples. Magnetic-bead technology allows parallel processing of 14 samples on the EZ1 Advanced XL or 24 samples on the EZ2 Connect.
产品特点
Features
- Fully automated cfDNA purification from up to 8 ml of plasma or serum
- Prefilled cartridges to ensure standardization and reduce human error
- High-quality cfDNA for downstream technologies like dPCR
- Run times from 35 to 70 minutes (1–8 ml plasma)
产品应用
Applications
cfDNA isolated using the easy-to-use and highly automated kit is ideal for analysis by downstream applications, including next-generation sequencing, qPCR and digital PCR (see figure Comparable yields in dPCR.
Comparison of cfDNA isolation with the EZ1&2 ccfDNA Kit, the Maxwell RSC ccfDNA Plasma Kit and the MagMAX Cell-Free DNA Isolation Kit show similar yields in dPCR. cfDNA from four different donors was isolated and wild-type BRAF amplified by dPCR using the QIAcuity.
The EZ1&2 ccfDNA Kit is highly suited for use in:
- Cancer research
- Non-invasive prenatal testing research (NIPT)
- Transplantation research
- cfDNA-based mutational and methylation analysis
- Biomarker discovery
Large-volume processing.
The EZ1&2 ccfDNA Kit processes of 1–8 ml plasma.
Higher degree of automation.
The EZ1&2 ccfDNA protocol is fully automated for all starting volumes up to 8 ml. cfDNA kits from Promega are fully automated for only the smallest starting volume of 1 ml and only partially automated for larger volumes.
Faster processing.
Run times for the EZ1&2 ccfDNA Kit run on the EZ2 Connect and Maxwell RSC ccfDNA Plasma Kit and the Maxwell system from Promega. Data are shown for processing 1 ml plasma sample.
cfDNA purification from 4 ml plasma.
Times indicated include manual pipetting steps, incubation and centrifugation times, transfer of eluates into elution tubes (mandatory for KingFisher), plasticware cleanup.
Linear DNA yields.
High yields of DNA are isolated from 2, 4 or 8 ml plasma. Yields were determined using the QuantiPlex Pro RGQ Kit (91 bp human fragment).
Flexible starting volumes.
Starting volumes were adjusted with PBS to allow processing with the standard protocols. Yields were determined using the QuantiPlexPro RGQ Kit (91 bp human fragment).
