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规格 |
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| Bacterial or Yeast Strain: | DH10B |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | One Shot™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| T1 Phage - Resistant (tonA): | Yes |
| Transformation Efficiency: | > 1x10^9 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
| Format: | One Shot |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
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储存 |
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| Store in ultra-cold freezer (-68 to -85°C). |
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描述 |
DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B-T1R genotype has the following features:
• tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones
DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3).
