规格
|
|
Label Type:
|
Biotin & Analogs |
|
Product Line:
|
Pierce™ |
|
Description:
|
Pierce Alkaline Phosphatase, Biotinylated |
|
Quantity:
|
1 mg |
储存
|
|
Store at or below -20°C. |
Thermo Scientific Pierce Biotinylated Proteins include biotin-labeled proteins (BSA), enzymes (HRP, AP) and fluorophores (FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.
Biotinylated AP is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
• Biotinylated enzymes—biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining
Biotin, also known as vitamin H, is a small molecule (MW 244.3) that is present in tiny amounts in all living cells and is critical for a number of biological processes. The valeric acid side chain of the biotin molecule can be derivatized in order to incorporate various reactive groups that are used to attach biotin to other molecules. In the context of immunohistochemistry (IHC), biotin is conjugated to antibodies or to the enzyme reporters used to detect target antigens.
Related Products
Pierce™ Bovine Serum Albumin, Biotinylated
Pierce™ Horseradish Peroxidase, Biotinylated
Pierce™ Biotin-Fluorescein Conjugate
Pierce™ Biotin
