The TurboCapture System uses unique oligonucleotide immobilization technology to provide a fast and simple procedure for high-throughput mRNA extraction. Cell lysates are added to the wells of a TurboCapture plate (96 or 384 wells), and mRNA is allowed to hybridize to the immobilized oligo-dT in each well. Contaminants are washed away, and the isolated mRNA is then either used directly in cDNA synthesis or eluted for use in downstream applications.
Plate-based mRNA purification in 96-well or 384-well format
Few protocol steps and minimal hands-on time
mRNA purification and cDNA synthesis in the same well
Standard plates compatible with robotic workstations
| Features | TurboCapture 96 mRNA Kit | TurboCapture 384 mRNA Kit |
|---|---|---|
| Applications | PCR, cDNA synthesis | PCR, cDNA synthesis |
| Elution volume | 80 µl | 20 µl |
| Format | 96-well plate | 384-well plate |
| Main sample type | Cultured cells | Cultured cells |
| Processing | Manual (centrifugation) | Manual (centrifugation) |
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | mRNA | mRNA |
| Sample amount | 80 µl | 20 µl |
| Stabilization | Yes | Yes |
| Technology | Oligonucleotide immobilization technology | Oligonucleotide immobilization technology |
| Time per run or per prep | 30 minutes – 1.5 hours | 30 minutes – 1.5 hours |
| Yield | Varies | Varies |
TurboCapture mRNA Kits are ideally suited for high-throughput gene expression analysis applications such as:
Real-time RT-PCR
Quantitative real-time RT-PCR
Screening of siRNAs by real-time RT-PCR
TurboCapture mRNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
