规格
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Assay Entry:
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Cell-based beta lactamase reporter gene |
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Cell Line:
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HCT-116 |
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Cell State:
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Dividing Cells |
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Druggable Target:
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Signaling Pathway |
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Product Line:
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CellSensor™ |
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System Type:
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CellSensor™ |
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Target Entry:
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Myc pathway, MYC |
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Detection Method:
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Fluorescent |
储存
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CellSensor® Myc-bla HCT116 cells are shipped on dry ice.
Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
The CellSensor™Myc-bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc. This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi against c-Myc and ICG-001, an inhibitor of the wnt-beta-catenin pathway. The expression of the mutant beta-catenin in HCT116 cells results in constitutive activation of beta-lactamase in this CellSensor™line, which can be knocked down by ICG-001 (Figure 1) or Myc RNAi (Figure 2).
