规格
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
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Key Functions:
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Constitutive Expression |
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Product Line:
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pcDNA™ |
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Constitutive or Inducible System:
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Constitutive |
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Delivery Type:
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Transfection |
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Promoter:
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CMV |
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Selection Agent (Eukaryotic):
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Hygromycin |
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Vector:
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pcDNA |
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Protein Tag:
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Untagged |
储存
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20 µg of this pcDNA™3.1/Hygro(-) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored. |
This pcDNA™3.1/Hygro(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
