规格
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Promoter:
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minP-NFκB |
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
储存
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Store in freezer (-5 to -30°C). |
The pNF-κB Tluc16-DD Vector is a transcriptional reporter vector designed to monitor the activation of NFκB protein and NFκB-regulated signal transduction pathways in mammalian cells. The vector encodes the smallest known luciferase, TurboLuc™16 (Tluc16) luciferase, as the reporter under the control of a combination of an optimized minimal core promoter and 5 tandem repeats of the NFκB transcriptional response element.
• Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
• Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
• Monitor activation of NF-κB protein and NF-κB-regulated signal transduction pathways in mammalian cells
Tluc16 luciferase is a 16 kDa, novel, intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild-type luciferase has been modified to reduce its size, increase its brightness, and enable its intracellular expression.
The pNF-κB Tluc16-DD luciferase expression vector also contains dual-destabilization (DD) technology that reduces accumulation of the Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay. A synthetic polyA terminator and a Transcriptional Pause Site (TPS) are included upstream of the NFκB response elements to minimize non-specific transcriptional read-through. The Tluc16 luciferase activity can be measured using the Thermo Scientific TurboLuc™ One-Step Glow Assay Kit.
