Clone REA482 recognizes domain 1 of the rat CD4 antigen, a single-pass type I membrane protein also known as T cell surface antigen T4/Leu-3 or W3/25 antigen. CD4 is a member of the immunoglobulin superfamily and is expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It has four immunoglobulin domains (D1 to D4). D1 and D3 resemble immunoglobulin variable (IgV) domains, and D2 and D4 resemble immunoglobulin constant (IgC) domains. CD4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell (APC). CD4 amplifies the signal generated by the TCR by recruiting the tyrosine kinase Lck, which is essential for activating many molecular components of the signaling cascade of an activated T cell. CD4 also interacts directly with MHC class II molecules on the surface of APCs.
Additional information: Clone REA482 displays negligible binding to Fc receptors.
W3/25, p55, Leu-3
Technical specifications
