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规格 |
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| Accession Number: | NM_001841 |
| Assay Entry: | Cell-based beta-arrestin recruitment |
| Cell Line: | U2OS |
| Cell State: | Division-Arrested Cells |
| Cytoplasmic Tail: | Tail from AVPR2 GPCR |
| Druggable Target: | GPCRs |
| Gene Symbol: | CNR2 |
| Population Status: | Single Cell Clone Origin |
| Product Line: | Tango™ |
| Reporter Gene: | BLA (Beta-Lactamase) |
| System Type: | Tango™ |
| Target Entry: | CNR2 |
| Detection Method: | Fluorescent |
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储存 |
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Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: o Tango™ CNR2-bla U2OS DA cells o LiveBLAzer™-FRET B/G Loading Kit, 70µg o Solution D o Tango™ CNR2-bla U2OS Assay Protocol o LiveBLAzer™-FRET B/G Loading Protocol o Certificate of Analysis The Tango™ CNR2-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
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描述 |
The Tango™ CNR2-bla U2OS DA (Division Arrested) cells and Tango™ CNR2-bla U2OS cells contain the human Cannabinoid Receptor 2 (CNR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ CNR2-bla U2OS cells and the Tango™ CNR2-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of CP-55940. In addition, Tango™ CNR2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
