规格
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Final Product Type:
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Transcriptome RNA |
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For Use With (Application):
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Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction |
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Purification Target:
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Transcriptome RNA |
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Sample Type:
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Total RNA |
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Format:
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Magnetic Bead |
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Isolation Technology:
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Magnetic Bead |
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High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
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System Type:
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RiboMinus |
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Product Line:
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RiboMinus™ |
储存
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The RiboMinus™ Human/Mouse Transcriptome Isolation Kit contains two modules; the RiboMinus™ Human/Mouse Probe Module and the Concentration Module. The RiboMinus™ Human/Mouse Probe Module contains the RiboMinus™ magnetic beads, the RiboMinus™ human/mouse probe, and binding buffer. The Concentration Module contains spin cartridges, wash tubes, recovery tubes, binding buffer, wash buffer, and RNase-free water. Store the RiboMinus" Human/Mouse Probe Module at +4·C. Store the Concentration Module at room temperature. All reagents are guaranteed stable for 6 months when properly stored. |
The RiboMinus™ Human/Mouse Transcriptome Isolation Kit constitutes a novel purification system that enriches the whole spectrum of mRNA species by depleting large ribosomal RNA molecules in a total RNA sample. The kit removes >95% of 18S and 28S rRNA molecules from human or mouse total RNA (Figure 1) without relying on current polyA+ purification methods. This increases the representation of mRNA species, including non-polyA-tailed, fragmented mRNA or other regulatory RNAs that cannot be purified via polyA-selection methods. Purified RNA is suitable for several downstream applications, including, library construction, RT-PCR, and microarray (Figure 2).
