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规格 |
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| No. of Wells: | 10 wells |
| Quantity: | 10 gels |
| Thickness (Metric): | 1 mm |
| Volume (Metric) Sample: | 25 µL |
| Gel Size: | Mini (8 cm x 8 cm) |
| For Use With (Equipment): | Mini Gel Tank, XCell SureLock™ Mini |
| Gel Percentage: | 10 % |
| Gel Type: | Zymogram Gel |
| Mode of Separation: | Molecular Weight |
| Separation Range: | 30 to 150 kDa (Tris-Glycine Buffer) |
| Shipping Condition: | Wet Ice |
| Substrate: | Gelatin |
| Substrate Type: | Protease Substrate |
| Well Design: | 1D Well |
| Product Line: | Novex™ |
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储存 |
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One box containing 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 4 months. |
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描述 |
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.
Formulation: Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and casein or gelatin as the substrate. All have a 4% stacking gel.
Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.
Recommended buffers: Use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer with these gels. Novex Zymogram Renaturing Buffer and Novex Zymogram Developing Buffer are also recommended.
