规格
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Cell Permeability:
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Cell-Permeant |
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Detection Method:
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Fluorescent |
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For Use With (Equipment):
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Fluorescence Microscope, Flow Cytometer, Microplate Reader |
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Indicator:
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pH Indicator |
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Indicator Properties:
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Fluorescent Dye-Based |
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Product Line:
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SNARF® |
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Shipping Condition:
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Room Temperature |
储存
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Store in freezer (-5 to -30°C). |
The cell-permeant ratiometric pH indicator SNARF®-5F 5-(and-6)-carboxylic acid, acetoxymethyl ester, acetate (pKa of ~7.2) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
