Clone REA474 recognizes the human CD307b antigen, a single-pass type I membrane protein, which is also known as Fc receptor-like protein 2 (FcRL2) or immunoglobulin receptor translocation-associated protein 4 (IRTA4). CD307b is a protein with immunomodulatory potential that is preferentially expressed by memory B cells in humans. It has two consensus ITIMs in addition to a putative ITAM sequence in its cytoplasmic domain. Coligation of CD307b with the B cell receptor (BCR) leads to tyrosine phosphorylation of its ITIM motifs and subsequent Src homology region 2 domain-containing phosphatase-1 recruitment to facilitate inhibition of BCR signaling. Mutational analysis indicates that the tyrosine residues in both inhibitory motifs of CD307b are required for complete inhibition of BCR signaling, whereas tyrosines in the putative activation motif are dispensable for signal modulation. These findings suggest a negative immunomodulatory function for CD307b in the regulation of memory B cells.
Additional information: Clone REA474 displays negligible binding to Fc receptors.
FCRL2, FcRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C
Technical specifications
