规格
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Crosslinker Type:
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Heterobifunctional |
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Labeling Method:
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Chemical Labeling |
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Product Line:
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Pierce™ |
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Reactive Moiety:
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Amine, Hydrazide, Maleimide |
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Spacer:
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Long |
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Solubility:
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DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
储存
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Upon receipt store desiccated at 4°C. This product is shipped at ambient temperature. |
Thermo Scientific Pierce KMUH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Features of KMUH:
• Reactive groups: maleimide and hydrazide
• Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
• Long (19.0A), sulfhydryl-to-aldehyde crosslinker with simple aliphatic spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
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