规格
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Assay:
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Kinase Binding Assay |
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Assay Entry:
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Biochemical binding/kinetic |
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Color:
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Far-Red |
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Excitation/Emission:
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340⁄665 |
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Label or Dye:
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Alexa Fluor™ 647 |
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Shipping Condition:
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Dry Ice |
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Target Entry:
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MAPK8, EGFR, RIPK2, MAPK9, EGFR T790M L858R, MAPK14, MAPK10, EGFR L861Q, EGFR L858R |
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Target Enzyme:
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Kinases (Multiple) |
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Technique:
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TR-FRET |
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Validated Kinases:
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EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2 |
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Detection Method:
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Fluorescent |
In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or 'tracer' such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Visit www.invitrogen.com/bindingassay to learn more and to see which kinases have been validated using this kinase tracer.
