产品介绍
Product Description
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规格 |
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| Bacterial or Yeast Strain: | DH10B |
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | DH10B, MAX Efficiency™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^9 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
| Format: | Tube(s) |
| Improves Plasmid Quality: | Yes |
| Species: | E. coli |
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储存 |
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Contains: • MAX Efficiency DH10B Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
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描述 |
MAX Efficiency DH10B Competent Cells are highly efficient E. coli, perfect for most applications. DH10B E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B genotype has the following features:
• lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity
