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规格 |
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| Bacterial or Yeast Strain: | DH5α |
| Cloning Method: | TOPO®-TA |
| Product Line: | One Shot™ |
| Vector: | TOPO-TA Cloning Vectors |
| Quantity: | 50 reactions |
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储存 |
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Box 1: • Topoisomerase I-activated pCR™2.1-TOPO® vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot® Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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描述 |
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO® Vectors include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1-TOPO®) or T7 and SP6 (pCR™II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
TOPO® TA Cloning® Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
