规格
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Assay Entry:
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Biochemical binding/kinetic |
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Kinase Group:
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Serine/Threonine Kinase |
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Species:
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Human |
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Target Entry:
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STK38 (NDR) |
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Molecular Weight:
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80.5 kDa |
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Gene Alias:
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NDR1, STK38 |
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Validated Application:
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Kinase Assay |
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Protein Tag:
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GST Tag |
储存
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Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
STK38 (also known as NDR) or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family that is regulated by Mob proteins. Fas and TNFα receptor stimulation activates human STK38 by promoting phosphorylation at the hydrophobic motif. NDR acts as a novel pro-apoptotic kinase, and is a key member of the RASSF1A/MST1 signaling cascade. STK38 is crucial for the fidelity of mitotic chromosome alignment in mammalian cells. STK38 is incorporated into the HIV-1 particles and is cleaved by the HIV-1 protease. STK38-driven centrosome duplication requires Cdk2 activity, and Cdk2-induced centrosome amplification is affected upon reduction of STK38 activity. Full-length recombinant human STK38 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
