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规格 |
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| Antibiotic Resistance Bacterial: | Ampicillin (AmpR) |
| Bacterial or Yeast Strain: | TOP10 |
| Cleavage: | EK (Enterokinase) Recognition Site |
| Cloning Method: | TOPO®-TA |
| Expression Mechanism: | Cell-Based Expression |
| Expression System: | E. coli |
| Product Line: | TOPO® |
| Constitutive or Inducible System: | Inducible |
| Inducing Agent: | IPTG |
| Promoter: | Trc and lacO |
| Selection Agent (Eukaryotic): | None |
| Vector: | pTrc |
| Protein Tag: | His Tag (6x), Xpress Epitope Tag |
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储存 |
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| The pTrcHis- and pTrcHis2-TOPO® TA Expression Kits each contain two boxes. The TOPO® TA box contains 200 ng of linearized, topoisomerase I-activated pTrcHis-TOPO® or pTrcHis2-TOPO® vector, sterile water, dNTPs, 10X PCR buffer, a control template and primers, primers for sequencing or PCR screening, and an expression control plasmid. The One Shot® TOP10 box contains twenty-one, 50-µl aliquots of chemically competent TOP10 E. coli, S.O.C. medium, and a supercoiled control plasmid. Store the TOPO TA Cloning® box at -20°C. Store the One Shot® box at -80°C. All reagents are guaranteed stable for 6 months when properly stored. |
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描述 |
The pTrcHis- and pTrcHis2-TOPO® TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from the trc promoter. The vector in each kit, pTrcHis-TOPO® or pTrcHis2-TOPO®, includes a minicistron element for enhanced translation efficiency of eukaryotic proteins in E. coli. Both vectors are provided linearized and activated with topoisomerase I for 5-minute TOPO® Cloning with >85% cloning efficiency. To simplify protein purification and detection, the pTrcHis-TOPO® and pTrcHis2-TOPO® vectors include the following features:
pTrcHis-TOPO®
• N-terminal Xpress™ epitope for detection with an Anti-Xpress™ Antibody
• N-terminal polyhistidine (6xHis) tag for purification using nickelchelating resin and detection with an Anti-HisG Antibody
• Enterokinase cleavage site for efficient removal of N-terminal fusion tags
pTrcHis2-TOPO®
• C-terminal c-myc epitope for detection with an Anti-myc Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
