规格
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Antibiotic Resistance Bacterial:
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Ampicillin (AmpR) |
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Cloning Method:
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Gateway® |
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Product Line:
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Gateway® |
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Constitutive or Inducible System:
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Inducible |
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Inducing Agent:
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IPTG |
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Promoter:
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T7 and lacO |
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Selection Agent (Eukaryotic):
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None |
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Vector:
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pET, pDEST |
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Protein Tag:
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His Tag (6x), V5 Epitope Tag |
储存
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The Champion™ pET-DEST42 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored. |
The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:
• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway® entry vector
