规格
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Substrate Properties:
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Chemical Substrate |
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Substrate Type:
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AP (Alkaline Phosphatase) Substrate |
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Description:
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Pierce PNPP Powder |
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Detection Method:
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Colorimetric |
储存
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Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.
Related Products
1-Step™ PNPP Substrate Solution
Pierce™ PNPP Substrate Kit
PNPP Substrate Tablets
