规格
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Cell Line:
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FreeStyle™ 293-F Cells |
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Cell State:
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Dividing Cells |
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Druggable Target:
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GPCRs |
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Gene Symbol:
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Gqo5 |
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Product Line:
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GeneBLAzer® |
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Reporter Gene:
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BLA (Beta-Lactamase) |
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System Type:
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GeneBLAzer™ |
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Detection Method:
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Fluorescent |
储存
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Shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use. |
GeneBLAzer® Gqo5-NFAT-bla FreeStyle™ 293F cells contain the chimeric G-protein Gqo5 stably integrated into the CellSensor® NFAT-blaFreeStyle™ 293F cell line. CellSensor® NFAT-bla FreeStyle™ 293F cells contain a beta-lactamase reporter gene under control of a stably integrated NFAT response element. Gqo5-NFAT-bla FreeStyle™ 293F is a parental cell line designed to be used for the construction of Gi/o-coupled GPCR beta-lactamase cellular assays. The Gi/o receptor of interest should be stably expressed in the cell line by expression from the selectable vector pcDNA™6.2-hygro-DEST (Cat. no. K1233). Gqo5-NFAT-bla FreeStyle™ 293F cells have been shown to respond to the agonist of a transiently expressed D2 receptor.
