The REAlease® CD4 (TIL) MicroBead Kit,human has been developed for positive selection of CD4+ tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.
The REAlease® CD4 (TIL) MicroBead Kit has been developed for the isolation of CD4+ tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid human tumors as well as xenotransplanted tumors of mice with a humanized immune system. CD4 is expressed on T helper cells, at a lower level on monocytes and dendritic cells, and at a very low level on hematopoietic progenitor cells.
The REAlease CD4 (TIL) MicroBead Kit is an indirect magnetic labeling system that allows to obtain cells free of MicroBeads and the REAlease Biotin Complex.
- Isolation of specific CD4+ tumor-infiltrating T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, for example, CD25, CD137 (4-1BB), CD223 (LAG-3), CD279 (PD1), or CD366 (TIM-3).
- Isolation of CD4+ tumor-infiltrating T cells which need to be label-free.
