Clone REA306 recognizes the human CD66b antigen, a glycosylphosphatidylinositol (GPI)–linked protein, which is also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8). CD66b is exclusively expressed on neutrophils and eosinophils and on leukocytes of chronic myeloid leukemia patients and bone marrow. Under normal conditions, neutrophils have minimal expression of CD66b, but incubation in vitro with inflammatory agonists rapidly increases this expression. Furthermore, expression of CD66b in eosinophils is enhanced in vivo in patients with a helminth infection, specifically with Schistosoma mansoni. The eosinophil expression of CD66b is also enhanced in vitro by incubation with activated lymphocytes or by brief incubation with interleukin 5 (IL-5). Crosslinking of CD66b on peripheral blood neutrophils mediates the release of IL-8 from intracellular storage.
Additional information: Clone REA306 displays negligible binding to Fc receptors.
CEACAM8, CD67, CGM6, NCA-95
Technical specifications
