规格
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Sample Type:
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Synthetic siRNA |
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Cell Type:
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Established Cell Lines, Primary Cells, Hard-to-Transfect Cells |
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High-throughput Compatibility:
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High-throughput Compatible |
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Serum Compatible:
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No |
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Shipping Condition:
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Wet Ice |
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Transfection Technique:
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Lipid-Based Transfection |
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Product Line:
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Oligofectamine™ |
储存
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Contains one vial (1 ml) Oligofectamine™ Reagent. Store at 4°C. Do not freeze. |
Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Transfection Reagent forms stable complexes with oligos, permitting efficient transfection into eukaryotic cells in a highly specific, yet nontoxic fashion. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.
Using Oligofectamine™ Transfection Reagent
Oligofectamine™ Reagent is easy to use because it provides a simple and fast protocol. Just dilute Oligofectamine™ Reagent, mix with oligonucleotide, and add to your cells. Oligofectamine™ Reagent requires nanomolar quantities of antisense oligonucleotide, reducing the amount of valuable oligonucleotide needed by up to a thousand-fold. This makes it ideal for high-throughput applications. In addition, Oligofectamine™ Reagent has also been shown to work for siRNA transfections. We recommend this reagent when performing RNAi knockdown experiments in HeLa cells. Please visit RNAi Central for more information.
