规格
|
|
Detection Method:
|
488nm channel |
|
Gel Compatibility:
|
Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels |
|
Number of Markers:
|
7 |
|
Product Line:
|
BenchMark™ |
|
Shipping Condition:
|
Dry Ice |
|
Size Range:
|
11 to 155 kDa |
|
System Type:
|
SDS-PAGE |
|
Molecular Weight:
|
155, 98, 63, 40, 32, 21, 11 kDa |
|
Stain Type:
|
bands conjugated to Alexa Fluor 488 |
储存
|
|
125 µl (25-50 applications) is provided in loading buffer consisting of Tris-HCl, SDS, Glycerol, and Coomassie® Blue G-250. Store at -20°C. Avoid repeated freezing and thawing. Stable for 6 months when properly stored. |
BenchMark Fluorescent Protein Standard consists of a wide molecular weight range of proteins (~11–155 kDa) conjugated to the fluorescent dye Alexa Fluor 488, allowing direct visualization using fluorescence. The proteins can be resolved into sharp bands by SDS-PAGE and visualized using a UV transilluminator, CCD camera with appropriate filters, or laser scanner systems.
Compare and view all other protein standards and ladders ›
Product features
• Visualization: fluorescence at 488 nm channel, maximum excitation at 493 nm and maximum emission at 516 nm
• Ready-to-use format: The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use
Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on fluorescent SDS-polyacrylamide gels and western blots
