规格
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Concentration:
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200 U⁄µl |
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Format:
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Tube(s) |
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No. of Reactions:
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200 reactions, 200 Reactions |
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Quantity:
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200 reactions |
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Sensitivity:
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Medium |
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Enzyme Function:
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RNA dependent DNA Polymerase |
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Size (Final Product):
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12.3 kb or less |
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Form:
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Frozen |
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Optimal Reaction Temperature:
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50° C |
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Reverse Transcriptase:
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SuperScript™ III |
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Ribonuclease H Activity:
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Reduced |
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Template:
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ssRNA |
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Product Line:
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SuperScript™ |
储存
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SuperScript® III Reverse Transcriptase (4 × 10,000 units total, at 200U/µL) is supplied with a vial (4 × 1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial (4 × 500 µL) of 100 mM DTT. Store at -20°C. |
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
