规格
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Exonuclease Activity:
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3' - 5', 5' - 3' |
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Hot Start:
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No |
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Polymerase:
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DNA Polymerase I |
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Shipping Condition:
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Wet Ice |
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Quantity:
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250 units |
储存
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Store in freezer (-5 to -30°C). |
DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.
• Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
• Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions
50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 µg/mL template/primer, 32 µM dTTP, 69 nM [3H]dTTP, and enzyme in 100 µL for 30 min at 37°C.
