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规格 |
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| Exonuclease Activity: | 3' - 5' |
| Fidelity (vs. Taq): | 2 X |
| GC-Rich PCR Performance: | High |
| Hot Start: | Built-In Hot Start |
| Overhang: | Blunt |
| Polymerase: | AccuPrime™ GC-Rich DNA Polymerase |
| Product Line: | AccuPrime™ |
| Quantity: | 200 reactions |
| Reaction Format: | Separate Components |
| Reaction Speed: | Standard |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
| Size (Final Product): | 5 kb or less |
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储存 |
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• 1 x 100 μL AccuPrime GC-Rich DNA Polymerase (2U/µL) • 1 x 1 mL 5X AccuPrime GC-Rich Buffer A • 1 x 1 mL 5X AccuPrime GC-Rich Buffer B • 1 x 1 mL 50 mM MgSO4 Store at -20°C. |
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描述 |
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:
• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.
