规格
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Binding Property:
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Standard Capacity, Affinity |
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Plate Blocking:
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Bovine Serum Albumin (BSA) |
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Plate Type:
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Detection Plate, Immunoassay, ELISA |
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Product Line:
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Pierce™ |
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Color:
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Black |
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Description:
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Pierce Nickel Coated Plates, Black, 96-Well |
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Detection Method:
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Fluorescent |
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Format:
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96-well plate |
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Surface Coating:
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Divalent Nickel, Metal Chelate |
储存
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Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C. |
Thermo Scientific Pierce Nickel Coated Black 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These black plates can be used with fluorescent detection methods.
Features of Pierce Nickel Coated Plates:
• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.
Related Products
Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well
