规格
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Final Product Type:
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Transcriptome RNA |
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For Use With (Application):
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Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction |
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Format:
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Magnetic Bead |
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Isolation Technology:
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Magnetic Bead |
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No. of Reactions:
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12 Reactions |
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Quantity:
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1 |
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Sample Type:
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Total RNA (Bacterial) |
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Yield:
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1 µg |
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High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
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Purification Target:
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Transcriptome RNA |
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Purification Time:
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1.25 hrs |
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Starting Material Amount:
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Up to 10 µg total RNA |
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System Type:
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RiboMinus |
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Product Line:
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RiboMinus™ |
储存
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The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored. |
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods. The RiboMinus™ Transcriptome Isolation Kits enable:
• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
