产品介绍
Product Description
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规格 |
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| Detection Method: | Fluorescent |
| Excitation/Emission: | 482⁄497 |
| Flow Cytometer Laser Lines: | 488 |
| Indicator: | Membrane Potential Probes |
| Indicator Properties: | Fluorescent Dye-Based |
| Label or Dye: | DiOC2(3) |
| No. of Reactions: | 100 |
| Product Line: | BacLight™ |
| Shipping Condition: | Wet Ice |
| For Use With (Equipment): | Flow Cytometer |
| Format: | Tube(s) |
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储存 |
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Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 µL of 500 µM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light. |
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描述 |
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
View additional information about all microbiology assays for flow cytometry.
