规格
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Label Type:
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Conjugating Groups |
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Label or Dye:
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Carboxyl Group |
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Product Line:
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Pierce™ |
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Reactive Moiety:
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Maleimide |
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Spacer:
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Mid-Length |
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Chemical Reactivity:
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Thiol |
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Solubility:
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Water |
储存
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Store desiccated at 4°C. Shipped at ambient temperature. |
Thermo Scientific Pierce EMCA is a carboxylic acid compound with a terminal maleimide group that can modify proteins and other molecules by EDC-mediated and sulfhydryl-targeted conjugation.
Features EMCA:
• Convert sulfhydryl groups to carboxyl groups for conjugation to amines via carbodiimide (EDC)
• Prepare maleimide-activated proteins using EDC to modify primary amines
• Maleimides react with—SH groups at pH 6.5-7.5, forming stable thioether linkages
EMCA (N-epsilon-maleimidocaproic acid) is a heterobifunctional crosslinker that reacts with sulfhydryl groups to present a terminal carboxyl group. Additionally, the carboxyl can be coupled to amine-containing molecules using the water-soluble carbodiimide EDC. The EDC reaction results in an intermediate ester, which can react with primary amines to form amide bonds. Thus, amine-containing molecules can be modified to contain sulfhydryl-reactive maleimides.
