规格
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Cell Permeability:
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Cell-Permeant |
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Detection Method:
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Fluorescent |
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For Use With (Equipment):
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Flow Cytometer, Microplate Reader |
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Product Line:
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LiveBLAzer™ |
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Shipping Condition:
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Room Temperature |
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Substrate:
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CCF4-AM |
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Substrate Properties:
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Chemical Substrate |
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Substrate Type:
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Beta-Lactamase Substrate |
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Quantity:
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20mg |
储存
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Store in freezer (-5 to -30°C). |
CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.
