规格
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Assay Entry:
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Biochemical binding/kinetic |
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Kinase Group:
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Serine/Threonine Kinase |
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Target Entry:
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ULK3 |
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Species:
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Human |
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Validated Application:
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Kinase Assay |
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Molecular Weight:
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54.3 kDa |
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Gene Alias:
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DKFZP434C131 |
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Protein Length:
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aa 44-end |
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Protein Tag:
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His Tag |
储存
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Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws. |
ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins. Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use
Related Product:
LanthaScreen® Eu Kinase Binding Assay
