产品介绍
Product Description
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规格 |
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| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope, Flow Cytometer |
| Format: | Tube(s) |
| No. of Reactions: | 60 |
| Excitation/Emission: | 495⁄519 |
| Flow Cytometer Laser Lines: | 488 |
| Host Species: | Mouse |
| Label or Dye: | Alexa Fluor™ 488 |
| Label Type: | Alexa Fluor Dyes |
| Purification Method: | Purified |
| Target Gene: | BrdU |
| Validated Application: | Flow Cytometry |
| Product Line: | APO-BrdU™ |
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储存 |
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Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 µL), 1 vial of BrdUTP (480 µL), 1 vial of anti-BrdU Ms mAb Alexa Fluor® 488 conjugate (350 µL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL). Store as directed. |
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描述 |
In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.
View a selection guide for all apoptosis assays for flow cytometry.
