规格
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Crosslinker Type:
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Heterobifunctional |
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Labeling Method:
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Chemical Labeling |
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Product Line:
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Pierce™ |
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Reactive Moiety:
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Maleimide, Isocyanate |
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Spacer:
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Short |
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Solubility:
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DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
储存
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Upon receipt store at 4°C protected from moisture. Product is shipped at ambient temperature. |
Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.
Features of PMPI:
• Reactive groups: maleimide and isocyanate
• Reactive towards: sulfhydryl and hydroxyl groups
• Short (8.7A), sulfhydryl-to-hydroxyl crosslinker with an aromatic spacer arm (noncleavable)
• Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
• Isocyanate reacts with non-aqueous hydroxyl groups to form carbamate linkage
• Excellent tool for conjugating hydroxyl-containing compounds such as steroids and vitamins
• Targets and reaction solutions must not contain primary amines
