规格
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Labeling Target:
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Proteins (Glycoproteins) |
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Label or Dye:
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Alexa Fluor™ 488, Alexa Fluor™ 555, Alexa Fluor™ 594, Alexa Fluor™ 647, Biotin, Oregon Green® 488, TMR (Tetramethylrhodamine) |
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Product Line:
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Click-iT™ |
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Shipping Condition:
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Room Temperature |
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Detection Method:
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Biotin-Based, Fluorescent |
储存
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Store in freezer (-5 to -30°C). |
The Click-iT® ManNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize cell surface sialic acid-containing glycoproteins. In step one, cultured cells are incubated with the azide-modified mannosamine (ManNAz). The azido-sugar is metabolically incorporated into cell surface sialic acid-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway,. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). These Click-iT® products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.
