产品介绍
Product Description
DNA sequence analysis and genotyping of biological samples using next-generation sequencing (NGS) platforms is often limited by the amount of available sample. The REPLI-g Advanced DNA Single Cell Kit has been specifically optimized for use in sensitive microarray and NGS applications, providing high uniformity, with minimal allelic dropout, when starting with single cells or purified genomic DNA. Buffers and reagents undergo a unique, controlled decontamination procedure to minimize contaminating DNA and ensure highly reliable results. Accurate amplification of genomes with negligible sequence bias and minimal genomic dropouts is achieved with innovative Multiple Displacement Amplification (MDA) technology.
产品特点
Features
- WGA with complete genome coverage from single eukaryotic cells or 1 ng genomic DNA
- Simple 3-step protocol requires only 2 hours and 20 minutes, start to finish
- Consistent yields of up to 25–35 µg DNA from 1 eukaryotic cell
- Includes cryo-protect reagent to minimize DNA damage during single cell capture, storage or shipping
产品应用
Applications
| Application | Instrumentation |
|---|---|
| Whole genome sequencing | Next-generation sequencing platforms* |
| Exome sequencing | |
| SNP genotyping arrays | Array platforms* |
| Array CGH | |
| qPCR/PCR technologies | Real-time PCR/PCR cyclers* |
| Sanger sequencing | Capillary sequencers* |
| Pyrosequencing | PyroMark (QIAGEN) |
References
1. Hosono, S. et al. (2003) Unbiased whole-genome amplification directly from clinical samples. Genome Res. 13, 954.