规格
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For Use With (Equipment):
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Flow Cytometer |
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Product Line:
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eBioscience™ |
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Quantity:
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100 tests |
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Format:
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Kit |
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Detection Method:
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Fluorescent |
Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.
Host
E.coli
Conjugate
Biotin
Purity
> 98% pure
Molecular Mass
35.8 kDa
Reported Application
Flow Cytometric Analysis
