General description
Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin subunit alpha-4 contains the C-terminal G domain, and although implicated in developmental regulation due to expression profiles, the biological function of laminin subunit alpha-4 is yet to be fully understood. Laminis as a whole play an important role in cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. High expression has been observed in adult heart, lung, ovary, small and large intestines, placenta, and liver, with lower levels observed in skeletal muscle, kidney, pancreas, testis, prostate, and rain.
~202 kDa observed under non-reduced conditions.
Immunogen
Recombinant protein corresponding to human Laminin subunit alpha-4.
Application
Research Sub Category
ECM Proteins
Anti-Laminin subunit alpha-4 Antibody, clone 3H2 detects level of Laminin subunit alpha-4 & has been published & validated for use in WB, IH, IP, ELISA.
Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Laminin subunit alpha-4 in basal cell carcinoma tissue.
ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)
Research Category
Cell Structure
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human serum tissue lysate (non-reduced)
Evaluated by Western Blot in human serum tissue lysate under non-reduced conditions.
Western Blot Analysis: A 1:50,000 dilution of this antibody detected Laminin subunit alpha-4 in 10 µg of human serum tissue lysate (non-reduced conditions).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3H2, monoclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001098676 |
| UniProt accession no. | Q16363 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LAMA4(3910) |
