General description
Ubiquitin-binding protein p62, also known as Sequestosome-1 (SQSTM1), is a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals. Defects in p62/SQSTM1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts
~60 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human SQSTM1.
Application
Anti-SQSTM1 Antibody, clone 20F1.1 is a highly specific mouse monoclonal antibody, that targets p62 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SQSTM1 in human thalamus and human cerebellum tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SQSTM1 in 10 µg of rat brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 20F1.1, monoclonal |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_001135770 |
| UniProt accession no. | Q13501 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SQSTM1(8878) |
